MCACS Home
Annual Meeting Home
Final Program
Past Meetings

Back to Annual Meeting Program


Optimization of aMSC-Engineered Craniofacial Repair: Nafibrous vs. Hybrid Scaffolds
Fabienne Gray, MD, Beatrice Dionigi, MD, Christopher G Turner, MD, MPH, Elliot C Pennington, MD, Azra Ahmed, BS, Thomas Darrah, PhD, Kaeuis A Faraj, PhD, David Zurakowski, PhD, Dario O Fauza, MD, PhD
Boston Children's Hospital and Harvard Medical School, Boston, MA

Background: Amniotic mesenchymal stem cell (aMSC)-engineered bone has been shown experimentally to be a viable alternative to perinatal craniofacial repair. Here, we sought to compare aMSC-derived bone grafts engineered from two fundamentally distinct biodegradable scaffolds in a leporine model of craniofacial reconstruction. Methods: After IACUC approval, six New Zealand rabbits underwent creation of 2 symmetrical, full thickness diploic nasal bone defects, each repaired with size-matched bone grafts engineered with allogeneic aMSCs. However, one scaffold derived from electrospun poly-L-lactic acid nanofibers, while the other consisted of a hybrid synthetic/natural tri-layer matrix encompassing porous and compacted Type-I collagen blended to poly-D,L-lactide-co-?-caprolactone polymer. Cell processing included phenotyping, nuclear labeling, and seeding optimized for each scaffold type. Constructs were maintained in osteogenic medium containing dexamethasone, ?-glycerophosphate and ascorbic acid, within a rotating bioreactor for 19-20 weeks. Animals were sacrificed 8 weeks post-implantation for multiple analyses. Statistical comparisons were by ANOVA and Levene’s F-test, as appropriate (P<0.05). Results: Closure of the defect was noted in all repairs. Constructs derived from nanofibers demonstrated statistically significant increase in bone mineralization when compared with hybrid scaffolds based on extracellular calcium levels, both in vitro and in vivo (P =0.02 and P =0.04, respectively), as well as based on radiodensity by 2D and 3D micro-CT scanning in vivo (P<0.01). Conclusion: Craniofacial repair with osseous grafts engineered from amniotic mesenchymal stem cells and electrospun nanofibers leads to enhanced mineralization when compared with a hybrid synthetic/natural scaffold. Craniofacial reconstruction with amniotic mesenchymal stem cell-engineered bone can be optimized by nanofibrous scaffolds.


Back to Annual Meeting Program

 



© 2023 Massachusetts Chapter of the American College of Surgeons. All Rights Reserved. Privacy Policy.